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METHOD a) Sample: We gave information about the objectives of our investigation to the female students of different Masters in Sexology, women associations and other entities. After that, we asked all these women for samples of urine an "ejaculated" fluid. We established an anonymous system by which the samples could reach us without us knowing from were they proceeded. If the women chose otherwise, they could also identify their samples. Once in the laboratory, the samples were filtered and numbered for the analysis. The complete sample consisted of 24 women, ages between 24 and 48. Six contributed with "ejaculation" samples, and the rest only with urine samples. b) Procedure : We did a Microparticle Enzyme Immunoassay (MEIA) of the pre and postorgasmic urine to detect PSA, using the Abbot AxSYM SYSTEM (1994, 1996), system and apparatus, and AxSYM PSA reactives. We also analyzed the liquid emitted in the moment of orgasm of our six samples to establish similarities and differences with the postorgasmic urine. Previously, we informed the women that they had to attain orgasm by manual self-stimulation, without having any contact with men's genitals, having previously washed their own genitals and a minimum of two days after the last sexual relationship. The biochemical parameter analyzed, considered by us the most specific and important to confirm our hypothesis, was the PSA. The importance of this parameter lies in the fact that it has only been found in prostatic tissue and in no other normal or pathological tissue. It has been isolated in seminal liquid (Wang et al., 1979, 1982; Papsidero et al., 1981; Nadji et al., 1981). It is a glycoprotein with a molecular weight of 33.000 to 34.000 daltons, with 7% of carbohydrates in it's molecule, that is found in the epithelial cells of prostatic ducts. It is observed in the prostatic secretion. On the other hand, women do not present PSA in urine or serum. RESULTS In 75% of the postorgasmic urine samples, we detected PSA. Applying a two-tailed Wilcoxon's pairs signet-ranks test, we found significant differences between pre and postorgasmic urine (p=0.0002). The mean PSA value in postorgasmic urine was 0.09 ng/ml when, theoretically, women do not have PSA. Out of the six women that gave us "ejaculation" samples, se found PSA in 100% of them being the average value 6.06 ng/ml. This extremely high figure was because one of the samples gave a value of 32 ng/ml of PSA (the analysis was repeated five times to confirm it) that can be caused by some kind of oncology pathology or maybe contamination of the sample by male semen. If we discard this figure, the mean value of PSA in ejaculation samples was of 0.82 ng/ml. In the postorgasmic urine of the subgroup of women that ejaculated, the levels of PSA were higher (mean value= 0.31 ng/ml, once discarded the extreme value sample previously mentioned) than in the rest of the samples of postorgasmic urine. Reprinted by permission of Dr. F. Cabello Santamarķa and the Scientific Committee of the 13th World Congress of Sexology.
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